Coding

Part:BBa_K3396007:Design

Designed by: yingqian Ye   Group: iGEM20_NUDT_CHINA   (2020-10-24)


Truncated Trim21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed restriction sites that are not compatible with biological parts standard. We also removed BsaI, BsmBI and SapI sites to allow Goden Gate assembly being used.


Source

The original Trim21 is expressed in human. We did the truncation based on the protein domain information given in Uniprot.

References